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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-34Z
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:5000-1:20,000, using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature.
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000.
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine).
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG F(c) fragment
SpecificityRecognizes Fc portion of human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against Human IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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