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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-34Z
Applications	Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-1:20,000, using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature.
	Western Blot: 1:500-1:2000
	Immunohistochemistry: 1:200-1:1000.
	Optimal dilutions to be determined by the researcher.
Label	Alkaline Phosphatase (Calf Intestine).
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human IgG F(c) fragment
Specificity	Recognizes Fc portion of human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against Human IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.