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You are here:Home » Antibodies » Abs to IgG F(abí)2 » Anti -IgG, F(ab')2 (HRP)

Anti -IgG, F(ab')2 (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-35H
ApplicationsSuitable for Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution:
ELISA: 1:30,000-1:150,000. Assayed against 1ug of Human IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG F(abí)2 fragment.
SpecificityRecognizes human IgG F(abí)2. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG, human IgG F(abí)2 and human serum. Does not crossreact with human IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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