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You are here:Home » Antibodies » Abs to IgG F(abí)2 » Anti -IgG, F(ab')2 (AP)

Anti -IgG, F(ab')2 (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-35J
Label Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Application(s) Suitable for immunoblotting (Western or Dot Blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilution(s)ELISA: 1:2000 to 1:9000. Assayed against 1ug of human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal titers for other applications should be determined by the researcher.
Storage Conditions Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenHuman IgG F(abí)2 fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F(abí)2 and human serum. No reaction was observed against suman IgG F(c).
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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