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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM, H&L, X-adsorbed (Biotin)

Anti -IgG, IgA, IgM, H&L, X-adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-35N
ApplicationsSuitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested
Recommended Dilution
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilution to be determined by the researcher.
Label Biotinamidocaproate N-Hydroxysuccinimide Ester(BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Companion ProductsA0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO
S7973-97A: Streptavidin (HRP)
Also AvailableI1904-04B: IgG, H&L (Biotin) Pab Gt xMo
I1904-06B: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-06G: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-09S: IgG, H&L (Biotin) Pab Gt xMo
I1904-09T: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-09U: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-14B: IgG, Fc (Biotin) Pab Gt xMo
I1904-14J: IgG, Fc, X-adsorbed (Biotin) Pab Gt xMo
I1904-19B: IgG, F(abí)2 (Biotin) Pab Gt xMo
I1904-25B: IgG, IgA, IgM H&L (Biotin) Pab Gt xMo
I1904-35N: IgG, IgA, IgM, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-67QIgG F(abí)2, X-adsorbed (Biotin) Pab Gt xMo
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long-term storage.. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG, IgA and IgM whole molecules
SpecificityRecognizes Mouse IgG, IgA and IgM, whole molecules (all subclasses, isotypes and chain combinations). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Species Crossreactivity: Does not crossreact with human serum protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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