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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM (HRP)

Anti -IgG, IgA, IgM (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-35T
ApplicationsSuitable for Western Blot, ELISA, Immunoelectrophoresis and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:2000:1:10,000
ELISA: 1:20,000-1:100,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG, IgA and IgM whole molecules
SpecificityRecognizes human IgG, IgA and IgM (all human immunoglobulin subclasses and chain combinations). Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG, human IgA and human IgM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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