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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-35X
Applications	Suitable for use in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:15000-1:75,000
	Western Blot: 1:2000-1:10,000
	Immunohistochemistry: 1:1000-1:5000
	Optimal dilutions to be determined by researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol.
Purity	Purified by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted crossreactivities.
Immunogen	Human kappa chain.
Specificity	Recognizes human IgG Kappa. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Crossreactivity (by ELISA)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.