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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG, Kappa (Biotin)

Anti -IgG, Kappa (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-35X
ApplicationsSuitable for use in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy. Other applications have not been tested.
Recommended DilutionsELISA: 1:15000-1:75,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol.
PurityPurified by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted crossreactivities.
ImmunogenHuman kappa chain.
SpecificityRecognizes human IgG Kappa. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Crossreactivity (by ELISA)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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