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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-40A
Applications	Suitable for use in Western Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:2000-1:10,000
	ELISA: 1:150,000
	Immunohistochemistry: 1:750-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Rabbit
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Purity	Purified by immunoaffinity chromatography followed by solid phase adsorption.
Immunogen	Rabbit IgG whole molecule
Specificity	Recognizes rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against human serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.