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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-40K
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4500
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRabbit
Concentration~1mg/ml
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
PurityPurified by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenRabbit IgG F(c) fragment
SpecificityRecognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rabbit IgG, rabbit IgG F(c) and rabbit serum. No reaction was observed against rabbit IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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