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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc, X-Adsorbed (HRP)

Anti -IgG, Fc, X-Adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-40P
ApplicationsSuitable for use in immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Other applications not tested.
Recommended DilutionELISA: 1:20,000 to 1:80,000 Standard ELISA.
Western Blot:1:2000 to 1:10,000
Immunohistochemistry: 1:500 to 1:2500.
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRabbit
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenRabbit IgG F(c) fragment
SpecificityRecognizes rabbit IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(abí)2 or Human Serum Proteins
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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