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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-43M
Applications	Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested
Recommended Dilutions
	ELISA: 1:4000 to 1:20,000, 30 minutes at RT.
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Rabbit IgG F(ab’)2 fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit
Serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.