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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	I1904-44A
Applications	Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	495nm
Emission Wavelength	528nm
Fluorochrome/Protein Ratio	2.7 moles FITC per mole of Goat IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (UV280)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Purity	Purified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.
Immunogen	Human lambda free chain.
Specificity	Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.