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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -IgG, Lambda (FITC)

Anti -IgG, Lambda (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IF
Catalog #I1904-44A
ApplicationsSuitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength 528nm
Fluorochrome/Protein Ratio2.7 moles FITC per mole of Goat IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.
ImmunogenHuman lambda free chain.
SpecificityRecognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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