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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1904-44C
Applications	Suitable for use in ELISA, Western and Dot blot, Immunoperoxidase electron microscopy, Immunohistochemistry, as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
	Other applications have not been tested.
Recommended Dilutions	ELISA: 1:16,000-1:64,000. Assayed against 1ug of human IgG
	in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (UV280)
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml 40-50% glycerol, ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human lambda light chain.
Specificity	Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Crossreactivity: Other human heavy or light chain isotypes <1%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.