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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -IgG, Lambda (HRP)

Anti -IgG, Lambda (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-44C
ApplicationsSuitable for use in ELISA, Western and Dot blot, Immunoperoxidase electron microscopy, Immunohistochemistry, as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Other applications have not been tested.
Recommended DilutionsELISA: 1:16,000-1:64,000. Assayed against 1ug of human IgG
in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml 40-50% glycerol, ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman lambda light chain.
SpecificityRecognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Crossreactivity: Other human heavy or light chain isotypes <1%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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