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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1904-44D
Label	Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Application(s)	Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Applications	Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:2000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (UV280)
Form	Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
Purity	Prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Immunogen	Human lambda
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-goat serum. Less than 1% crossreactivity against other human heavy or light chain isotypes.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.