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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG (HRP)

Anti -IgG (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-44S
ApplicationsSuitable for use in Immunoblot (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:60,000-1:300,000. Use ABTS as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Conjugated to horseradish peroxidase (HRP). Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG gamma chain.
SpecificityRecognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, human IgG and human serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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