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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (FITC)

Anti -IgG, Fc (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Catalog #I1904-44V
ApplicationsSuitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended DilutionOptimal dilution determined by the researcher.
Label Fluorescein isothiocyanate (FITC) (MW: 390D)
Absorption Wavelength 495 nm
Emission Wavelength 528 nm
Fluorochrome/Protein Ratio3.1 moles FITC per mole of Rabbit IgG
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH .2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 40% glycerol. labeled with FITC MW
PurityPurified by affinity chromatography.
ImmunogenHuman IgG F(abí)2
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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