Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH Gel
Catalog #I1904-44Y
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:1600 to 1:16,000 using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine) (MW: 140kD)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase
(AP).
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenHuman IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links