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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-47X
Applications	Suitable for use Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.
Recommended Dilution	ELISA: 1:40,000-1:200,000
	Optimal dilutions to be determined by the researcher.
Label	Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10–20 BAC molecules per Goat IgG molecule
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Rat
Concentration	~2mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide, 40% glycerol. Conjugated to Biotin.
Purity	Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.
Immunogen	Rat IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.