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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-47Z
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:5000. assayed against 1ug of rat IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase
(AP).
PurityPrepared by immunoaffinity chromatography followed by solid phase adsorption.
ImmunogenRat IgG F(c) fragment
SpecificityRecognizes rat IgG F(c) fragment. Does not crossreact with rat IgG F(abí)2. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rat IgG, rat IgG F(c) and rat serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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