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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM, H&L (HRP)

Anti -IgG, IgA, IgM, H&L (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-48M
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase electron microscopy, and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:5000-1:25,000
Western Blot: 1:500-1:3000
Immunohistochemistry: 1:500-1:1500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerisol, 40% glycerol. labeled with horseradish peroxidase (HRP).
PurityPrepared from polyspecific antiserum by immunoaffinity chromatography.
ImmunogenRat IgG, IgA and IgM whole molecules.
SpecificityRecognizes rat IgG, IgA, IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. Suitable for the detection of all rat immunoglobulin isotypes ad chain combinations.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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