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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-48U
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:300,000-1:1,400,000
	Western Blot: 1:30,000-1:150,000
	Immunohistochemistry: 1:15,000-1:75,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~3mg/ml (UV280)
Form	Supplied as a liquid in 0.02 M PBS, pH 7.2, 0.01% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Rat IgG whole molecule.
Specificity	Recognizes rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, rat IgG and rat serum. No reaction was observed against human serum proteins
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.