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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-49B
ApplicationsSuitable for use in Immunoblotting, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications have not been tested.
Recommended DilutionELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10–20 BAC molecules per Rabbit IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration1.5 mg/ml (UV280)
FormSupplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Biotin conjugated.
PurityPurified by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
ImmunogenRat IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against rat IgG F(ab’)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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