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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-49B
Applications	Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications have not been tested.
Recommended Dilution	ELISA: 1:5000-1:20,000
	Optimal dilutions to be determined by the researcher.
Label	Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10–20 BAC molecules per Rabbit IgG molecule
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	1.5 mg/ml (UV280)
Form	Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Biotin conjugated.
Purity	Purified by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
Immunogen	Rat IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against rat IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.