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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH Gel
Catalog #I1904-49C
ApplicationsSuitable for Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000 to 1:5000. Assayed against 1ug of Rat IgG in a standard capture ELISA.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration1.5 mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPrepared by immunoaffinity chromatography followed by solid phase adsorption.
ImmunogenRat IgG F(c) fragment
SpecificityRecognizes Rat IgG F(c) fragment. Does not crossreact with Rat IgG F(abí)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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