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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-49D
LabelAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution ELISA 1:500-1:3,000. 1ug of rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenRat IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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