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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc, X-adsorbed (FITC)

Anti -IgG, Fc, X-adsorbed (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IF
Catalog #I1904-49M
ApplicationsSuitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.8moles FITC per mole of goat IgG, F(abí)2.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (MW
PurityPurified by immunoaffinity chromatography.
ImmunogenRat IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(abí)2 or Bovine, Horse and Human Serum Proteins
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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