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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc, X-adsorbed (AP)

Anti -IgG, Fc, X-adsorbed (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-49Q
ApplicationsSuitable for Immunoblot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:1000 to 1:5000 using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris -HCl, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase.
PurityPurified by immunoaffinity chromatography.
ImmunogenRat IgG F(c) fragment. Label
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(abí)2 or Bovine, Horse, and Human Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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