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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	I1904-50P1
Applications	Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Label	Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength	550nm
Emission Wavelength	570nm
Fluorochrome/Protein Ratio	4.0 moles TRITC per mole of Goat IgG F(ab’)2
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide. Labeled with TRITC.
Purity	Purified by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Immunogen	Rat IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.