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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, H&L (Biotin)

Anti -IgG, H&L (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-57B
ApplicationsSuitable for use in Westrn Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionELISA: 1:30,000-1:150,000; Assayed against 1ug of porcine IgG in a standard capture ELISA using S7973-97A (Streptavidin (HRP)) and A0030 (ABTS Stable Liquid Substrate) for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourcePorcine
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules/goat IgG molecule
PurityPurified by immunoaffinity chromatography.
ImmunogenPorcine IgG whole molecule
SpecificityRecognizes porcine IgG, H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, porcine IgG and porcine serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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