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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-57N
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:6500-1:27,000; Assayed against 1ug of porcine IgG in a standard capture ELISA using ABTS as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourcePorcine
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenPorcine IgG F(c) fragment.
SpecificityRecognizes porcine IgG Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, porcine IgG, porcine IgG F(c) and porcine serum. No reaction was observed against porcine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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