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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-57P
Applications	Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Label	Fluorescein isothiocyanate (FITC) (Molecular weight: 390D)
Absorption Wavelength	495nm
Emission Wavelength	528nm
Fluorochrome/Protein Ratio	~2 moles FITC per mole of Rabbit IgG
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Porcine
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol.
Purity	Purified by immunoaffinity chromatography using porcine IgG coupled to agarose beads followed by solid phase adsorption(s).
Immunogen	Porcine IgG F(ab’)2 fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, porcine IgG, porcine IgG F(ab’)2 and porcine serum. No reaction was observed against porcine IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.