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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-57T
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-1:50,000; 1ug of porcine IgG F(ab’)2 was used in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT.
	Western Blot: 1:1000-1:10,000
	Immunohistochemistry: 1:500-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Porcine
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Porcine IgG F(ab')2 fragment.
Specificity	Recognizes porcine IgG F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, porcine IgG F(ab')2 and porcine serum. No reaction was seen against porcine IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.