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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH Gel
Catalog #I1904-57U
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry Other applications not tested.
Recommended DilutionELISA: 1:1000-1:4000. 1.0ug of Porcine IgG was used in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% Glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenPorcine IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(c) and Swine Serum. No reaction was observed against Swine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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