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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-59L
Label	Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength	495 nm
Emission Wavelength	528 nm
Fluorochrome/Protein Ratio	2.3 moles FITC per mole of Rabbit IgG
Application(s)	Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested.
Recommended Dilution(s)	Not specified. Optimal titers for applications should be determined by the researcher.
Storage Conditions	Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at-20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Sheep
Concentration	1.5 mg/ml (UV280)
Form	FITC conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free 0.01% sodium azide
Purity	Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.
Immunogen	Sheep IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.