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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-59M
ApplicationsSuitable for use in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.
Recommended DilutionsELISA: 1:15,000-1:60,000 using 1ug sheep IgG in a sandwich ELISA.
Optimal dilution determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per Rabbit IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceSheep
Concentration~1.5mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
ImmunogenSheep IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, sheep IgG, sheep IgG F(c) and sheep serum. No reaction was observed against sheep IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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