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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH Gel

Catalog #	I1904-59R
Label	Horseradish Peroxidase (HRP)
Application(s)	Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
Recommended Dilution(s)	Immunohistochemistry: 1:500 to 1:5000 on tissue sections
	ELISA: 1:5000-1:50,000
	Western Blot: 1:5000-1:50,000
	Optimal titers for other applications should be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Sheep
Concentration	1.0 mg/ml (UV280)
Form	Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerosal (addition of sodium azide not recommended). Conjugated to horseradish peroxidase (HRP).
Purity	Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption.
Immunogen	Sheep IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2 or Human Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.