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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-59U
Applications	Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 1:1000-1:5000
	ELISA: 1:10,000-1:50,000
	Optimal dilutions to be determined by the researcher.
Excitation Wavelength	495nm
Emmission Wavelength	528nm
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Sheep
Concentration	~1.5 mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).. F/P Ratio: 2.8 moles FITC/mole Rabbit IgG
Purity	Purified by immunoaffinity chromatography. F/P Ratio: 2.8 moles DTAF/mole Rabbit IgG
Immunogen	Sheep IgG F(ab’)2 fragment.
Specificity	Recognizes sheep IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.