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You are here:Home » Antibodies » Abs to IgG F(abí)2 » Anti -IgG, F(ab')2 (FITC)

Anti -IgG, F(ab')2 (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Catalog #I1904-59U
ApplicationsSuitable for use in Immunofluorescence and FLISA. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:1000-1:5000
FLISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Excitation Wavelength 495nm
Emmission Wavelength 528nm
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceSheep
Concentration~1.5 mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).. F/P Ratio: 2.8 moles FITC/mole Rabbit IgG
PurityPurified by immunoaffinity chromatography. F/P Ratio: 2.8 moles DTAF/mole Rabbit IgG
ImmunogenSheep IgG F(abí)2 fragment.
SpecificityRecognizes sheep IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, sheep IgG, sheep IgG F(abí)2 and sheep serum. No reaction was observed against sheep IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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