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You are here:Home » Antibodies » Abs to IgG F(abí)2 » Anti -IgG, F(ab')2 (Biotin)

Anti -IgG, F(ab')2 (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-59V
ApplicationsSuitable for use in ELISA, Immunoblot, Immunomicroscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:40,000-1:160,000
Optimal dilutions to be determined by the researcher.
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio 10-20 BAC molecules per Rabbit IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceSheep
Concentration1.5 mg/ml (UV280)
FormSupplied as a liquid in 0.02 M PBS, pH 7.2, 10 mg/ml BSA, (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Biotin conjugated.
PurityPurified by immunoaffinity chromatography.
ImmunogenSheep IgG F(abí)2 fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(abí)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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