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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-59Z
Applications	Suitable for ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended Dilution	Optimal dilution determined by the researcher.
Label	FITC MW: 390D
Absorption Wavelength	495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio	3.1moles FITC per mole of Rabbit IgG
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Rabbit
Concentration	~1.0 mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10 mg/ml BSA, IgG and Protease free,
0.05% Sodium Azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Sheep IgG F(ab’)2 fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c) or Human Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.