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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG H&L, X-adsorbed (Biotin)

Anti -IgG H&L, X-adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IH
Catalog #I1904-61F
ApplicationsSuitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionsELISA: 1:10,000 to 1:50,000 using Peroxidase Conjugated Streptavidin and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceSheep
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitution: Reconstitute with 1ml sterile dH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSheep IgG, whole molecule. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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