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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG H&L, X-adsorbed (HRP)

Anti -IgG H&L, X-adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IH
Catalog #I1904-61G
ApplicationsSuitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:10,000
ELISA: 1:10,000-1:50,000. Assayed against 1ug of sheep IgG in a standard sandwich ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.
Immunohistochemistry: 1:500-1:2500
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceSheep
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSheep IgG whole molecule.
SpecificityRecognizes sheep IgG H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, sheep IgG and sheep serum. No reaction was observed against chicken, guinea pig, Golden Syrian Hamster, equine, human, mouse, rabbit and rat serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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