Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Donkey Affinity Purified E B IH

Catalog #	I1904-61G
Applications	Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000-1:10,000
	ELISA: 1:10,000-1:50,000. Assayed against 1ug of sheep IgG in a standard sandwich ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.
	Immunohistochemistry: 1:500-1:2500
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Donkey
Source	Sheep
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Sheep IgG whole molecule.
Specificity	Recognizes sheep IgG H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, sheep IgG and sheep serum. No reaction was observed against chicken, guinea pig, Golden Syrian Hamster, equine, human, mouse, rabbit and rat serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.