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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-62H
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Label	Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength	550nm
Emission Wavelength	570nm
Fluorochrome/Protein Ratio	2.2 moles TRITC per mole of Goat IgG F(ab’)2
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	IgG, H&L (Biotin)
Concentration	1.5 mg/ml (by UV absorbance at 280 nm)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide. Labeled with TRITC.
Purity	Purified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Immunogen	Sheep IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.