Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (TRITC)

Anti -IgG, Fc (TRITC)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1904-62H
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
LabelTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength 550nm
Emission Wavelength 570nm
Fluorochrome/Protein Ratio 2.2 moles TRITC per mole of Goat IgG F(abí)2
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceIgG, H&L (Biotin)
Concentration1.5 mg/ml (by UV absorbance at 280 nm)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide. Labeled with TRITC.
PurityPurified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenSheep IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links