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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, H&L (HRP)

Anti -IgG, H&L (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IH
Catalog #I1904-63
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoperoxidase Electron Microscopy. Other applications have not been tested.
Recommended DilutionELISA: 1:150,000
Western Blot: 1:3000-1:15,000
Immunohistochemistry: 1:1000-1:5000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceSheep
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Conjugated to horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenSheep IgG whole molecule.
SpecificityRecognizes sheep IgG. Assayed against 1ug of sheep IgG in a standard capture ELISA. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, sheep IgG and sheep serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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