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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Heavy Chain

Anti -IgG, Heavy Chain

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
The most common feature of plasmacytomas, and certain non-Hodgkin’s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Catalog #I1904-65A1
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:500-1:1000 for 30 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified
ImmunogenIgG isolated from a pool of normal human sera. Cellular Localization: Cytoplasmic
SpecificityRecognizes human IgG heavy chain at ~50kD. Does not crossreact with alpha (IgA), epsilon (IgE), mu (IgM), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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