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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH

The most common feature of plasmacytomas, and certain non-Hodgkin’s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Catalog #	I1904-65A1
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Formalin/paraffin): 1:500-1:1000 for 30 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control	Tonsil
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity	Purified
Immunogen	IgG isolated from a pool of normal human sera. Cellular Localization: Cytoplasmic
Specificity	Recognizes human IgG heavy chain at ~50kD. Does not crossreact with alpha (IgA), epsilon (IgE), mu (IgM), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.