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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG, Kappa (FITC)

Anti -IgG, Kappa (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1904-66P
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Label Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.0 moles FITC per mole of Rabbit IgG
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration1.0 mg/ml (UV280)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label with Fluorescein isothiocyanate (FITC). Reconstitute with sterile dH2O or 40-50% glycerol for long term storage.
PurityPrepared by immunoaffinity chromatography.
ImmunogenMouse kappa free chain
SpecificityRecognizes Mouse kappa free chain. Crossreactivity against other Mouse heavy or light chain isotypes is < 1%. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-rabbit serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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