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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH Gel

Catalog #	I1904-66R
Applications	Suitable for Immunoblot (Western and Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:2000-1:5000
	ELISA: 1:50,000
	Immunohistochemistry: 1:500-1-2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease free), 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile distilled water or sterile 40-50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse k (kappa) light chain
Specificity	Recognizes mouse IgG k (kappa) light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgG and mouse serum. Crossreactivity against other mouse heavy and light chain isotypes (confirmed by ELISA): 1%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.