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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG, Kappa (AP)

Anti -IgG, Kappa (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-66S
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA (Capture): 1:1000-1:2000. Assayed against 1ug of mouse IgG.
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine) (MW: 140,000D)
Storage and StabilityStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one year from date of opening product.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse k (kappa light chain)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorptions against a panel of mouse IgG lambda, IgM lambda and IgA lambda proteins was performed. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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