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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -IgG, Lambda (AP)

Anti -IgG, Lambda (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-66W
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:1000-1:2000. Coat with 1ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase
Source: Calf Intestine
Molecular Weight: 140,000D
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
PurityPrepared by immunoaffinity chromatography followed by solid phase adsorption.
ImmunogenMouse Lambda light chain
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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