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You are here:Home » Antibodies » Abs to IgG2 » Anti -IgG2b (Texas Red)

Anti -IgG2b (Texas Red)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Catalog #I1904-67H
ApplicationsSuitable for use in FLISA (Fluorescent), Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionFLISA (Fluorescent): 1:10,000-1:50,000
Immunofluorescence: 1:100-1:5000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 596 nm
Emission Wavelength 620 nm
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red® conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG2b
HostRabbit
SourceMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Redô. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG2b heavy chain
SpecificityRecognizes mouse IgG2b. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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