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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1904-67P
Applications	Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:20,000 - 1:100,000
	Western Blot: 1:2,000 - 1:10,000
	Immunohistochemistry: 1:1,000 -1:5,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Purity	Purified by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove unwanted reactivities, pepsin digestion and chromatographic separation.
Immunogen	Mouse IgG whole molecule
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), or bovine, horse, human, rabbit, rat, and sheep serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.