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You are here:Home » Antibodies » Abs to IgG (H&L) » Anti -IgG, H&L, X-Adsorbed

Anti -IgG, H&L, X-Adsorbed

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-67P
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:20,000 - 1:100,000
Western Blot: 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 -1:5,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove unwanted reactivities, pepsin digestion and chromatographic separation.
ImmunogenMouse IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), or bovine, horse, human, rabbit, rat, and sheep serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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