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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG F(abí)2, X-adsorbed (Biotin)

Anti -IgG F(abí)2, X-adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-67Q
ApplicationsSuitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested
Recommended Dilutions
ELISA: 1:4000-1:100,000.1.0 ug of mouse IgG was used in a standard capture ELISA using S7973-97A (Streptavidin (HRP)) and A0030 (ABTS Stable Liquid Substrate 1.46mM for HRPO) as a substrate for 30 minutes at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography
ImmunogenMouse IgG F(abí)2 fragment
SpecificityRecognizes mouse IgG F(abí)2 fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, mouse IgG F(abí)2 and mouse serum. Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgGF(c). Species Crossreactivity: Does not crossreact with bovine, equine, human, rabbit, rat and sheep serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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