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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1904-67Q
Applications	Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested
Recommended Dilutions
	ELISA: 1:10,000-1:40,000, assayed against 1ug of mouse IgG in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 min at RT.
	Optimal dilution to be determined by the researcher.
Biotin/Protein Ratio	10-20 BAC molecules per goat IgG molecule
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C .Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG F(ab’)2 fragment
Specificity	Recognizes Mouse IgG F(ab’)2 fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, mouse IgG F(ab’)2 and mouse serum. Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgGF(c). Species Crossreactivity: Does not crossreact with bovine, equine, human, rabbit, rat and sheep serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.