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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG H&L, X-adsorbed (HRP)

Anti -IgG H&L, X-adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-67R
ApplicationsSuitable for use in Immunoblot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:20,000; 1.0ug of mouse IgG was used in a standard capture ELISA with ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (A0030) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostRabbit
SourceMouse
Concentration~0.5 mg/ml
FormSupplied as a lypohilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Label: Horseradish Peroxidase (HRP)
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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