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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-67R
Applications	Suitable for use in Immunoblot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:20,000; 1.0ug of mouse IgG was used in a standard capture ELISA with ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (A0030) as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Rabbit
Source	Mouse
Concentration	~0.5 mg/ml
Form	Supplied as a lypohilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Label: Horseradish Peroxidase (HRP)
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG whole molecule
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.